EDS, when combined with these imaging tools, can provide spatially resolved elemental analysis from areas as small as 1 nanometer in diameter (STEM). In SEM, the analysis volume is larger and ranges in volume from perhaps 0.1 to 3 microns. The impact of the electron beam on the sample produces x-rays that are characteristic of the elements present on the sample. EDS analysis can be used to determine the elemental composition of individual points, line scans or to map out the lateral distribution of elements from the imaged area.
Ideal Uses of EDS
Elemental composition of small areas using SEM/TEM imaging
Defect identification/mapping
Medical device chemical characterization and equivalency testing via ISO 10993:19
Strengths
Quick “first look” compositional analysis
Versatile, inexpensive, and widely available
Quantitative for some samples (flat, polished, homogeneous)
Limitations
Generally, semi quantitative analysis is possible
Sample size must be compatible with SEM/TEM
Samples must be vacuum compatible (not ideal for wet organic materials)
Analysis (and coating) may restrict subsequent surface analysis
Numerous elemental peak overlaps are possible, careful review of the spectra is required
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